Paraburkholderia atlantica is the main rhizobial symbiont of Mimosa spp. in ultramafic soils in the Brazilian Cerrado biome

To evaluate the occurrence, characterization and identity of nodulating bacteria in symbiosis with Mimosa spp. ultramafic massif Barro Alto, Goiás state, Brazil. Nodules from field grown M. somnians claussenii were sampled for isolation situ detection using microscopy. Isolates characterized their nodulation capacity on pudica common bean, tolerance to Ni culture medium. Bacteria also partially identified by 16S rRNA gene sequences. In addition, recA, gyrB, nodC nifH genes five representative isolates sequenced phylogenetic studies. indicated exclusive presence Paraburkholderia sp. within nodules. This identification was confirmed most analysis All as able effectively nodulate pudica, but those tested bean produced ineffective Inoculation tests showed that these improved growth absence nitrogen. None tolerance. The concatenated rRNA, recA sequences demonstrated selected closely related P. atlantica. is first report present nodules plants naturally growing soils. These results suggest this may be a factor considered part programs restore soils Alto areas degraded mining activity.